The newest version of FaSTR DNA from STRmix includes improved artefact detection and filtering, the ability to group replicates, and the addition of a number of contributors (NoC) estimation tool for Y-STR profiles.

FaSTR DNA 1.3 combines an intuitive, user-friendly graphical interface with easily understandable and laboratory-customizable rules to rapidly analyze raw DNA data generated by capillary electrophoresis instruments and multiplex DNA profiling kits, and assign number of contributor (NoC) estimates.

Along with improvements introduced in the previous version of the software, such as known artefact detection and optional new viewing modes for scanpoint view and individual channel zoom, FaSTR DNA 1.3 features the ability to export a consensus reference profile, detect absence of primer flare in all sample types, add notes to EPGs and snips for PDF reports, create a subset of an analyzed project for better case management, and filter values in the peaks table columns.

FaSTR DNA 1.3 also introduces detection of cross channel artefacts, improved known artefact detection (to include positional artefacts), additional customization options for sample reports and exports, and additional pull-up detection information with the display of pull-up indicators for parent peaks.

FaSTR DNA works by applying a set of highly configurable rules to streamline the analysis of STR DNA profiles. In some instances, DNA analysis is more complex and automated heuristics (analysis rules) alone may not be able to resolve all profiles. Where the intervention of a DNA analyst is required, FaSTR DNA readily provides all details related to possible ambiguity of a peak (including stutter type, stutter ratio, composite stutter, pull-up proportion, N/shoulder peak, peak morphology, stochastic homozygous threshold, heterozygote imbalance, known artefact information etc.) and signals a requirement for the DNA analyst to make a decision. It then records and highlights changes made by the analyst. 

The algorithms used for analysis in FaSTR DNA are conceptually adapted from the proven approach of OSIRIS analysis software (National Center for Biotechnology Information).

When used in conjunction with STRmix™ (which resolves mixed DNA profiles previously regarded as too complex or degraded to interpret) and DBLR™ (which enables users to calculate any kinship relationship conceivable, undertake fast database searches, visualize the value of DNA mixture evidence, and determine whether there is a common donor to profiles), FaSTR DNA delivers an end-to-end analysis, interpretation, and intelligence solution, completing the full workflow.

Prior to releasing FaSTR DNA 1.3, the STRmix Team completed additional compatibility testing of the software with .fsa files generated using the latest security patched data collection software provided by Thermo Fisher Scientific on the 3500 Genetic Analyzer and SeqStudio Flex Genetic Analyzer. The files tested were generated using the GlobalFiler™ and PowerPlex Y23 kits respectively. These files can be successfully opened and analyzed in all commercially released versions of FaSTR™ DNA. 

For more information about FaSTR™ DNA 1.3, visit https://www.strmix.com/fastr.