Akonni Biosystems

TruTip DNA Extraction Kit

Akonni Biosystems today announced the availability of a new family of "smart tip" extraction products for rapidly purifying human genomic deoxyribonucleic acid (gDNA) on the Hamilton Microlab STAR line of robotic liquid handling workstations. Starting from fresh or frozen blood, buffy coat, and soon tissue or cells, these new TruTip kits extract superior quality gDNA simultaneously from eight to 96 samples in just 57 minutes. The system does not require a magnetic head, vacuum manifold, centrifuge, or other space-consuming or expensive capital equipment. Polymerase chain reaction(PCR) inhibition due to bead carryover is also eliminated since there are no magnetic beads required, as are errors due to clogging typically caused by vacuum manifolds.

The TruTip family of products uses a porous nucleic acid binding matrix embedded in a pipette tip, and extracts DNA or ribonucleic acid (RNA) using well-understood chaotropic salt chemistry. This n ew kit for gDNA completes the entire extraction process (including a fully automated onboard heat incubation step) in less than an hour (20 minutes for heat incubation and 37 minutes for extraction) and is over 250 percent faster than most traditional high-throughput approaches. TruTip also delivers "gold-standard-equivalent" yields and equal or superior purity and reproducibility, by comparison.

TruTip gDNA Kits for use on the STAR line of workstations are now available for early-access customers. TruTip applications for extraction of gDNA from saliva collected using the Oragene1DISCOVER and Oragene1Dx Collection Kit from DNA Genotek, Inc., a subsidiary of OraSure Technologies, Inc., will be available soon, as well as for swab and Scope mouthwash input sample types. TruTip gDNA Kits can be used with a variety of back-end analytical tools, including qPCR, real time PCR, digital PCR, microarrays, and mid-multiplex bead-based systems, as well as traditional and next-generation sequencing systems.

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